1H NMR Signals from Urine Excreted Protein Are a Source of Bias in Probabilistic Quotient Normalization
 

1H NMR Signals from Urine Excreted Protein Are a Source of Bias in Probabilistic Quotient Normalization

Normalization to account for variation in urinary dilution is crucial for interpretation of urine metabolic profiles. Probabilistic quotient normalization (PQN) is used routinely in metabolomics but is sensitive to systematic variation shared across a large proportion of the spectral profile (>50%). Where ¹H nuclear magnetic resonance (NMR) spectroscopy is employed, the presence of urinary protein can elevate the spectral baseline and substantially impact the resulting profile. Using ¹H NMR...

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