BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > Educational resources > Journal club
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 02-09-2018, 11:21 AM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 23,134
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default Morphology and magnetic structure of the ferritin core during iron loading and releasing by magnetooptical and NMR methods.

Morphology and magnetic structure of the ferritin core during iron loading and releasing by magnetooptical and NMR methods.

Morphology and magnetic structure of the ferritin core during iron loading and releasing by magnetooptical and NMR methods.

ACS Appl Mater Interfaces. 2018 Feb 08;:

Authors: Koralewski M, Balej?íková L, Mitroova Z, Pochylski M, Baranowski M, Kopcansky P

Abstract
The ferritin is a protein, which serves as a storage and transportation capsule for iron inside living organisms. Continuous charging protein with iron and releasing it from the ferritin is necessary to assure proper management of this important ions within organism. From other side synthetic ferritin have great potential for biomedical and technological applications. In this work the behavior of ferritin during the processes of iron loading and releasing was examined using multiplicity of experimental technique. The quality of protein's shell was monitored using circular dichroism, whereas the average size and its distribution was estimated from dynamic light scattering and TEM images, respectively. Because of the magnetic behavior of the iron mineral, number of magnetooptical methods were used to gain information on the iron core of the ferritin. Faraday rotation and magnetic linear birefringence studies provides evidence that the iron loading and the iron release processes are not symmetrical. The spatial organization of the mineral within the protein's core changes depending on whether the iron was incorporated to, or removed from the ferritins shell. Magnetic optical rotatory dispersion spectra exclude contribution of Fe(II)-composed mineral, whereas joined magnetooptical and NMR results indicate that no mineral with high magnetization appear at any stage of the loading/release process. These findings suggest that the iron core of loaded/released ferritin consists of single-phase i.e. ferrihydrite. Presented results demonstrate usefulness of emerging magnetooptical methods in biomedical research and applications.


PMID: 29417811 [PubMed - as supplied by publisher]



More...
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
The Crystal Structure of a Maxi/Mini-Ferritin ChimeraReveals Guiding Principles for the Assembly of Protein Cages
The Crystal Structure of a Maxi/Mini-Ferritin ChimeraReveals Guiding Principles for the Assembly of Protein Cages http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b00312/20170721/images/medium/bi-2017-00312m_0003.gif Biochemistry DOI: 10.1021/acs.biochem.7b00312 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/II86hGTUL8I More...
nmrlearner Journal club 0 07-22-2017 05:57 AM
Correlating Synthetic Methods, Morphology, Atomic-Level Structure, and Catalytic Activity of Sn-? Catalysts
From The DNP-NMR Blog: Correlating Synthetic Methods, Morphology, Atomic-Level Structure, and Catalytic Activity of Sn-? Catalysts Wolf, P., et al., Correlating Synthetic Methods, Morphology, Atomic-Level Structure, and Catalytic Activity of Sn-? Catalysts. ACS Catalysis, 2016. 6(7): p. 4047-4063. http://dx.doi.org/10.1021/acscatal.6b00114
nmrlearner News from NMR blogs 0 07-13-2016 10:22 PM
Unraveling the core-shell structure of ligand-capped Sn/SnOx nanoparticles by surface-enhanced nuclear magnetic resonance, Mossbauer, and X-ray absorption spectroscopies
From The DNP-NMR Blog: Unraveling the core-shell structure of ligand-capped Sn/SnOx nanoparticles by surface-enhanced nuclear magnetic resonance, Mossbauer, and X-ray absorption spectroscopies Protesescu, L., et al., Unraveling the core-shell structure of ligand-capped Sn/SnOx nanoparticles by surface-enhanced nuclear magnetic resonance, Mossbauer, and X-ray absorption spectroscopies. ACS Nano, 2014. 8(3): p. 2639-48. http://www.ncbi.nlm.nih.gov/pubmed/24494636
nmrlearner News from NMR blogs 0 11-21-2014 09:04 PM
[NMR paper] Solution and Solid State NMR Approaches To Draw Iron Pathways in the Ferritin Nanocage.
Solution and Solid State NMR Approaches To Draw Iron Pathways in the Ferritin Nanocage. Solution and Solid State NMR Approaches To Draw Iron Pathways in the Ferritin Nanocage. Acc Chem Res. 2013 Sep 3; Authors: Lalli D, Turano P Abstract Ferritins are intracellular proteins that can store thousands of iron(III) ions as a solid mineral. These structures autoassemble from four-helix bundle subunits to form a hollow sphere and are a prototypical example of protein nanocages. The protein acts as a reservoir, encapsulating iron as ferric oxide...
nmrlearner Journal club 0 09-05-2013 01:07 PM
[NMR paper] Solution NMR structure investigation for releasing mechanism of neocarzinostatin chro
Solution NMR structure investigation for releasing mechanism of neocarzinostatin chromophore from the holoprotein. Related Articles Solution NMR structure investigation for releasing mechanism of neocarzinostatin chromophore from the holoprotein. J Biol Chem. 2005 Mar 25;280(12):11340-6 Authors: Takashima H, Yoshida T, Ishino T, Hasuda K, Ohkubo T, Kobayashi Y Holo-neocarzinostatin (holo-NCS) is a complex protein carrying the anti-tumor active enediyne ring chromophore by a scaffold consisting of an immunoglobulin-like seven-stranded...
nmrlearner Journal club 0 11-24-2010 11:14 PM
[NMR paper] Iron uptake by ferritin: NMR relaxometry studies at low iron loads.
Iron uptake by ferritin: NMR relaxometry studies at low iron loads. Related Articles Iron uptake by ferritin: NMR relaxometry studies at low iron loads. J Inorg Biochem. 1998 Sep;71(3-4):153-7 Authors: Vymazal J, Brooks RA, Bulte JW, Gordon D, Aisen P Twenty ferritin samples were prepared at pH 6.5 with average loadings of 0-89 Fe atoms per molecule. Nuclear magnetic relaxation times T1 and T2 were measured at 3 degrees C, 23 degrees C, and at 37 degrees C and at field strength from 0.025 to 1.5 T. The field dependence, temperature dependence,...
nmrlearner Journal club 0 11-17-2010 11:15 PM
NMR structure of the first extracellular domain of corticotropin releasing factor rec
NMR structure of the first extracellular domain of corticotropin releasing factor receptor 1 (ECD1-CRF-R1) complexed with a high affinity agonist. Related Articles NMR structure of the first extracellular domain of corticotropin releasing factor receptor 1 (ECD1-CRF-R1) complexed with a high affinity agonist. J Biol Chem. 2010 Sep 15; Authors: Grace CR, Perrin MH, Gulyas J, Rivier JE, Vale WW, Riek R The corticotropin releasing factor (CRF) peptide hormone family members coordinate endocrine, behavioral, autonomic and metabolic responses to...
nmrlearner Journal club 0 09-17-2010 04:14 PM
Installation of GROMACS 3.3.1 on Dell Inspiron 6400 with Fedora Core 6, Test 3, Dual Core processor
This is not really a "hard-core NMR topic" but it could be useful for people who try to complement dynamics data from NMR relaxation experiments with MD simulations. I had really hard time trying to install the newer versions of Gromacs 3.3 and 3.3.1 on my laptop (Dell Inspiron 6400 Dual Core processor) . The laptop used to run Suse 10.1 that was recently replaced with Fedora Core 6 Test 3 (that finally supports Intel integrated mobile 945 video cards). With both OS, Gromacs 3.3.x could be installed, however, its sub-program "genion" failed (the program never ends while consuming 100%...
administrator Molecular Dynamics programs 0 10-04-2006 11:50 AM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 12:14 PM.


Map