A 1H-15N NMR study of human c-Ha-ras protein: biosynthetic incorporation of 15N-label
 

A 1H-15N NMR study of human c-Ha-ras protein: biosynthetic incorporation of 15N-labeled amino acids.

Related Articles A 1H-15N NMR study of human c-Ha-ras protein: biosynthetic incorporation of 15N-labeled amino acids.

J Biomol NMR. 1992 Jan;2(1):71-82

Authors: Yamasaki K, Muto Y, Ito Y, Wälchli M, Miyazawa T, Nishimura S, Yokoyama S

A 1H-15N NMR study was performed on the GDP-bound form of a truncated human c-Ha-ras oncogene product (171 amino acid residues). Resonance cross peaks of the backbone amide 1H-15N nuclei of a uniformly 15N-labeled protein were observed with heteronuclear single-quantum coherence spectroscopy (HSQC). In order to resolve overlapping cross peaks, selective 15N-labeling of one or two types of amino acid residues (Ala, Arg, Asx, Glx, Gly, His, Ile, Leu, Lys, Met, Phe, Ser, Thr, Tyr and/or Val) was carried out using appropriate E. coli mutant strains. By this procedure, all the backbone 1H-15N cross peaks were classified into amino acid types.

PMID: 1422147 [PubMed - indexed for MEDLINE]



Source: PubMed