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Default 1H-13C Separated Local Field Spectroscopy of Uniformly 13C Labeled Peptides and Prote

1H-13C Separated Local Field Spectroscopy of Uniformly 13C Labeled Peptides and Proteins


Publication year: 2010
Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 1 July 2010

Eugene C., Lin , Chin H., Wu , Yuan, Yang , Christopher V., Grant , Stanley J., Opella

By incorporating homonuclear decoupling on both the 1H and 13C channels it is feasible to obtain high-resolution two-dimensional separated local field spectra of peptides and proteins that are 100% labeled with 13C. Dual-PISEMO (Polarization Inversion Spin Exchange Modulated Observation) can be performed as a conventional two-dimensional experiment, or with windowed detection as a one-dimensional experiment that offers flexibility as a building block for Shiftless and other multidimensional triple-resonance experiments with the inclusion of 15N irradiation. The triple-resonance MAGC probe used to perform these experiments at 500 MHz is described.


Source: Journal of Magnetic Resonance
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