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Default 19F NMR evidence for interactions between the c-AMP binding sites on the c-AMP recept

19F NMR evidence for interactions between the c-AMP binding sites on the c-AMP receptor protein from E. coli.

Related Articles 19F NMR evidence for interactions between the c-AMP binding sites on the c-AMP receptor protein from E. coli.

FEBS Lett. 1991 May 20;283(1):127-30

Authors: Hinds MG, King RW, Feeney J

The 19F NMR spectra of 3-fluorotyrosine containing c-AMP receptor protein (CRP) from E. coli have been recorded in the presence of increasing amounts of c-AMP. One of the signals (from Tyr B) shifts upfield by 0.6 ppm in the presence of excess c-AMP and shows both slow and fast exchange behaviour during the titration. This is evidence for interactions between the two c-AMP binding sites on the CRP dimer leading to different dissociation rate constants (less than or equal to 75 s-1; greater than or equal to 350 s-1) for complexes containing one and two c-AMP molecules.

PMID: 1645291 [PubMed - indexed for MEDLINE]



Source: PubMed
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