[NMR paper] Comparison of Targeted (HPLC) and Nontargeted (GC-MS and NMR) Approaches for the Detection of Undeclared Addition of Protein Hydrolysates in Turkey Breast Muscle.
Comparison of Targeted (HPLC) and Nontargeted (GC-MS and NMR) Approaches for the Detection of Undeclared Addition of Protein Hydrolysates in Turkey Breast Muscle.
Related ArticlesComparison of Targeted (HPLC) and Nontargeted (GC-MS and NMR) Approaches for the Detection of Undeclared Addition of Protein Hydrolysates in Turkey Breast Muscle.
Foods. 2020 Aug 08;9(8):
Authors: Wagner L, Peukert M, Kranz B, Gerhardt N, Andrée S, Busch U, Brüggemann DA
Abstract
The adulteration of fresh turkey meat by the undeclared addition of protein hydrolysates is of interest for fraudsters due to the increase of the economic gain by substituting meat with low cost ingredients. The aim of this study was to compare the suitability of three different analytical techniques such as GC-MS and 1H-NMR with HPLC-UV/VIS as a targeted method, for the detection of with protein hydrolysates adulterated turkey meat. For this, turkey breast muscles were treated with different plant- (e.g., wheat) and animal-based (e.g., gelatin, casein) protein hydrolysates with different hydrolyzation degrees (15-53%: partial; 100%: total), which were produced by enzymatic and acidic hydrolysis. A water- and a nontreated sample (REF) served as controls. The data analyses revealed that the hydrolysate-treated samples had significantly higher levels of amino acids (e.g., leucine, phenylalanine, lysine) compared with REF observed with all three techniques concordantly. Furthermore, the nontargeted metabolic profiling (GC-MS and NMR) showed that sugars (glucose, maltose) and/or by-products (build and released during acidic hydrolyses, e.g., levulinic acid) could be used for the differentiation between control and hydrolysates (type, degrees). The combination of amino acid profiling and additional compounds gives stronger evidence for the detection and classification of adulteration in turkey breast meat.
[NMR paper] Sensory assessment of fish and chicken protein hydrolysates - evaluation of NMR metabolomics profiling as a new prediction tool.
Sensory assessment of fish and chicken protein hydrolysates - evaluation of NMR metabolomics profiling as a new prediction tool.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Sensory assessment of fish and chicken protein hydrolysates - evaluation of NMR metabolomics profiling as a new prediction tool.
J Agric Food Chem. 2020 Mar 02;:
Authors: Steinsholm S, Oterhals Ĺ, Underhaug J, Mage I, Malmendal A, Aspevik T
Abstract
Nuclear magnetic resonance...
[NMR paper] 1H-NMR analysis of turkey egg-white lysozyme and comparison with hen egg-white lysozy
1H-NMR analysis of turkey egg-white lysozyme and comparison with hen egg-white lysozyme.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles 1H-NMR analysis of turkey egg-white lysozyme and comparison with hen egg-white lysozyme.
Eur J Biochem. 1993 Jul 15;215(2):255-66
Authors: Bartik K, Dobson CM, Redfield C
The complete main chain and approximately 75% of the side chain 1H-NMR assignments of the 129-residue protein, turkey...
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NMR for Nontargeted Screening of Melamine in Baby Foods
NMR for Nontargeted Screening of Melamine in Baby Foods
The recent melamine crisis in China has pointed out a serious deficiency in current food control systems, namely, they specifically focus on selected known compounds. This targeted approach allowed the presence of melamine in milk products to be overlooked for a considerable time. To avoid this in the future, nontargeted NMR screening is proposed.
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Comparison of Targeted (HPLC) and Nontargeted (GC-MS and NMR) Approaches for the Detection of Undeclared Addition of Protein Hydrolysates in Turkey Breast Muscle.
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