15N-Detected TROSY NMR experiments to study large disordered proteins in high-field magnets
 

15N-Detected TROSY NMR experiments to study large disordered proteins in high-field magnets

Intrinsically disordered regions (IDRs) of proteins are critical in the regulation of biological processes but difficult to study structurally. Nuclear magnetic resonance (NMR) is uniquely equipped to provide structural information on IDRs at atomic resolution; however, existing NMR methods often pose a challenge for large molecular weight IDRs. Resonance assignment of IDRs using ^(15)N^(D)-detection was previously demonstrated and shown to overcome some of these limitations. Here, we improve...

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