NMR paper 13C NMR signal detection of iron-bound cyanide ions in ferric cyanide complexes of he

		BioNMR > Educational resources > Journal club
[NMR paper] 13C NMR signal detection of iron-bound cyanide ions in ferric cyanide complexes of he

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

View First Unread

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-24-2010, 08:49 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,174

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

13C NMR signal detection of iron-bound cyanide ions in ferric cyanide complexes of he

13C NMR signal detection of iron-bound cyanide ions in ferric cyanide complexes of heme proteins.

Related Articles 13C NMR signal detection of iron-bound cyanide ions in ferric cyanide complexes of heme proteins.

J Am Chem Soc. 2002 May 29;124(21):5936-7

Authors: Fujii H

13CN ion appears to have the greatest potential to probe the heme environment of the ferric heme proteins; however, a resonance of the iron-bound (13)CN ion in ferric heme proteins has not yet been located. We show here the first detection of (13)C NMR signals of the iron-bound (13)CN for heme proteins and their model complexes in an unexpectedly large upfield region. This study demonstrates that the (13)C NMR signal of the iron-bound (13)CN is a sensitive probe to study the nature of the proximal ligand in ferric heme protein.

PMID: 12022815 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Dynamic Nuclear Polarization-Enhanced Solid-State NMR of a 13C-Labeled Signal Peptide Bound to Lipid-Reconstituted Sec Translocon Dynamic Nuclear Polarization-Enhanced Solid-State NMR of a 13C-Labeled Signal Peptide Bound to Lipid-Reconstituted Sec Translocon Lenica Reggie, Jakob J. Lopez, Ian Collinson, Clemens Glaubitz and Mark Lorch http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja209378h/aop/images/medium/ja-2011-09378h_0002.gif Journal of the American Chemical Society DOI: 10.1021/ja209378h http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/e6Ae3MMc0OU	nmrlearner	Journal club	0	11-09-2011 06:44 AM
[NMR tweet] ~ RT ? kmobs: You everyone signal saying I have crappy on ? Tmobile, signal I get in an Nuclear magnetic resonance ... http://b1t.it/kd6 ~ RT ? kmobs: You everyone signal saying I have crappy on ? Tmobile, signal I get in an Nuclear magnetic resonance ... http://b1t.it/kd6 Published by BUGabundoPT2ENG (BUGabundo PT 2 Eng) on 2011-05-20T11:03:03Z Source: Twitter	nmrlearner	Twitter NMR	0	05-20-2011 11:26 AM
[NMR paper] Iron uptake by ferritin: NMR relaxometry studies at low iron loads. Iron uptake by ferritin: NMR relaxometry studies at low iron loads. Related Articles Iron uptake by ferritin: NMR relaxometry studies at low iron loads. J Inorg Biochem. 1998 Sep;71(3-4):153-7 Authors: Vymazal J, Brooks RA, Bulte JW, Gordon D, Aisen P Twenty ferritin samples were prepared at pH 6.5 with average loadings of 0-89 Fe atoms per molecule. Nuclear magnetic relaxation times T1 and T2 were measured at 3 degrees C, 23 degrees C, and at 37 degrees C and at field strength from 0.025 to 1.5 T. The field dependence, temperature dependence,...	nmrlearner	Journal club	0	11-17-2010 11:15 PM
[NMR paper] 1H NMR study of dynamics and thermodynamics of acid-alkaline transition in ferric hem 1H NMR study of dynamics and thermodynamics of acid-alkaline transition in ferric hemoglobin of a midge larva (Tokunagayusurika akamusi). Related Articles 1H NMR study of dynamics and thermodynamics of acid-alkaline transition in ferric hemoglobin of a midge larva (Tokunagayusurika akamusi). Biochim Biophys Acta. 1998 Jun 11;1385(1):89-100 Authors: Koshikawa K, Yamamoto Y, Kamimura S, Matsuoka A, Shikama K One of the components of hemoglobin from the larval hemolyph of Tokunagayusurika akamusi possesses naturally occurring substitution at the...	nmrlearner	Journal club	0	11-17-2010 11:06 PM
[NMR paper] 2D NMR of paramagnetic metalloenzymes: cyanide-inhibited horseradish peroxidase. 2D NMR of paramagnetic metalloenzymes: cyanide-inhibited horseradish peroxidase. Related Articles 2D NMR of paramagnetic metalloenzymes: cyanide-inhibited horseradish peroxidase. J Biomol NMR. 1991 Jul;1(2):175-90 Authors: de Ropp JS, Yu LP, La Mar GN Two-dimensional (2D) proton NMR correlation spectroscopy, COSY, and nuclear Overhauser spectroscopy, NOESY, have been used to explore the applicability of these methods for the moderately large (42 KDa), paramagnetic cyanide-inhibited derivative of horseradish peroxidase, HRP-CN. The target...	nmrlearner	Journal club	0	08-21-2010 11:16 PM
[NMR paper] NMR study of the active site of resting state and cyanide-inhibited lignin peroxidase NMR study of the active site of resting state and cyanide-inhibited lignin peroxidase from Phanerochaete chrysosporium. Comparison with horseradish peroxidase. Related Articles NMR study of the active site of resting state and cyanide-inhibited lignin peroxidase from Phanerochaete chrysosporium. Comparison with horseradish peroxidase. J Biol Chem. 1991 Aug 15;266(23):15001-8 Authors: de Ropp JS, La Mar GN, Wariishi H, Gold MH One- and two-dimensional 1H NMR spectroscopy has been used to probe the active site of the high spin ferric resting...	nmrlearner	Journal club	0	08-21-2010 11:12 PM
[NMR paper] NMR study of the active site of resting state and cyanide-inhibited lignin peroxidase NMR study of the active site of resting state and cyanide-inhibited lignin peroxidase from Phanerochaete chrysosporium. Comparison with horseradish peroxidase. Related Articles NMR study of the active site of resting state and cyanide-inhibited lignin peroxidase from Phanerochaete chrysosporium. Comparison with horseradish peroxidase. J Biol Chem. 1991 Aug 15;266(23):15001-8 Authors: de Ropp JS, La Mar GN, Wariishi H, Gold MH One- and two-dimensional 1H NMR spectroscopy has been used to probe the active site of the high spin ferric resting...	nmrlearner	Journal club	0	08-21-2010 11:12 PM
[NMR paper] 2D NMR and structural model for a mitochondrial signal peptide bound to a micelle. 2D NMR and structural model for a mitochondrial signal peptide bound to a micelle. Related Articles 2D NMR and structural model for a mitochondrial signal peptide bound to a micelle. Biochemistry. 1990 Oct 23;29(42):9872-8 Authors: Karslake C, Piotto ME, Pak YK, Weiner H, Gorenstein DG The 19 amino acid signal peptide of rat liver aldehyde dehydrogenase, possessing a lysine substitution for an arginine and containing 3 extra amino acid residues at the C terminus, was studied by two-dimensional NMR in a dodecylphosphocholine micelle. In this...	nmrlearner	Journal club	0	08-21-2010 11:04 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off