Related Articles13C-NMR relaxation study on mobility of the DNA-binding arm of HU.
J Biochem. 1994 Nov;116(5):1153-5
Authors: Kakuta Y, Hojo H, Aimoto S, Tanaka I, Hikichi K
The mobility of the DNA-binding arm of HU protein was studied by 13C-NMR spectroscopy. The correlation times tau c of Phe47C alpha in the body and Gly60C alpha in the arm of HU were determined for HU and HU-DNA complex. The value of tau c of Phe47C alpha is 2-4 times larger than that of Gly60C alpha irrespective of the presence or absence of DNA. These results show that Gly60C alpha undergoes more rapid motion than Phe47C alpha. The increase in correlation time on addition of DNA is greater for Gly60C alpha than for Phe47C alpha. This suggests that the addition of DNA influences more significantly the motion of Gly60C alpha than that of Phe47C alpha. These results are in accord with the X-ray result, in which the top part of the arm is not visible. Gly60C alpha in the arm is thus more mobile than Phe47C alpha in the body, and the mobility of Gly60C alpha is reduced by the DNA binding.
[NMR paper] A solid-state NMR study of molecular mobility and phase separation in co-spray-dried
A solid-state NMR study of molecular mobility and phase separation in co-spray-dried protein-sugar particles.
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Eur J Pharm Sci. 2005 May;25(1):105-12
Authors: Suihko EJ, Forbes RT, Apperley DC
Molecular mobility and physical form of co-spray-dried sugar-lysozyme formulations were evaluated. Co-spray-dried trehalose:lysozyme and sucrose:lysozyme formulations in 1:9, 1:1 and 9:1 ratios (w:w) were stored at 0% RH and 75%...
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[NMR paper] A 15N NMR mobility study on the dicalcium P43M calbindin D9k and its mono-La3+-substi
A 15N NMR mobility study on the dicalcium P43M calbindin D9k and its mono-La3+-substituted form.
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Biochemistry. 2002 Apr 23;41(16):5104-11
Authors: Bertini I, Carrano CJ, Luchinat C, Piccioli M, Poggi L
Calbindin D(9k) is a dicalcium binding protein consisting of two helix-loop-helix EF-hand motifs joined together by a flexible linker region where one metal ion can bind to each of the two loops. A proline residue at position 43 in...
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Study of effect of molecular mobility in chromatophore membranes of the bacterium E.
Study of effect of molecular mobility in chromatophore membranes of the bacterium E. shaposhnikovii on processes of photoinduced electron transport using the NMR-spin-echo method with isotope substitution and dehydration.
Related Articles Study of effect of molecular mobility in chromatophore membranes of the bacterium E. shaposhnikovii on processes of photoinduced electron transport using the NMR-spin-echo method with isotope substitution and dehydration.
Biochemistry (Mosc). 2010 Apr;75(4):423-7
Authors: Chamorovsky CS, Chamorovsky SK, Knox PP
...
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[NMR paper] Ligand binding alters the backbone mobility of intestinal fatty acid-binding protein
Ligand binding alters the backbone mobility of intestinal fatty acid-binding protein as monitored by 15N NMR relaxation and 1H exchange.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles Ligand binding alters the backbone mobility of intestinal fatty acid-binding protein as monitored by 15N NMR relaxation and 1H exchange.
Biochemistry. 1997 Feb 25;36(8):2278-90
Authors: Hodsdon ME, Cistola DP
The backbone dynamics of the liganded (holo) and unliganded (apo) forms of Escherichia...
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[NMR paper] Ligand binding alters the backbone mobility of intestinal fatty acid-binding protein
Ligand binding alters the backbone mobility of intestinal fatty acid-binding protein as monitored by 15N NMR relaxation and 1H exchange.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles Ligand binding alters the backbone mobility of intestinal fatty acid-binding protein as monitored by 15N NMR relaxation and 1H exchange.
Biochemistry. 1997 Feb 25;36(8):2278-90
Authors: Hodsdon ME, Cistola DP
The backbone dynamics of the liganded (holo) and unliganded (apo) forms of Escherichia...
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[NMR paper] 7Li NMR relaxation study of Li+ binding in human erythrocytes.
7Li NMR relaxation study of Li+ binding in human erythrocytes.
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Biochemistry. 1993 Dec 14;32(49):13490-8
Authors: Rong Q, Espanol M, Mota de Freitas D, Geraldes CF
We used 7Li NMR spin-lattice (T1) and spin-spin (T2) relaxation time measurements to investigate the binding of Li+ in human red blood cell (RBC) suspensions. In RBCs containing 1.4 mM Li+, the intracellular 7Li NMR T2 relaxation value (0.30 +/- 0.03 s) was much smaller than the corresponding T1 value...
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[NMR paper] Loop mobility in a four-helix-bundle protein: 15N NMR relaxation measurements on huma
Loop mobility in a four-helix-bundle protein: 15N NMR relaxation measurements on human interleukin-4.
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Biochemistry. 1992 Nov 3;31(43):10431-7
Authors: Redfield C, Boyd J, Smith LJ, Smith RA, Dobson CM
15N NOE, T1, and T2 measurements have been carried out on uniformly 15N-labeled human interleukin-4. Analysis of the results in terms of order parameters (S2) shows that although the helical core of this four-helix-bundle protein...
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[NMR paper] 1H-NMR study of heme propanoate mobility in the active site of myoglobin from Galeorh
1H-NMR study of heme propanoate mobility in the active site of myoglobin from Galeorhinus japonicus.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles 1H-NMR study of heme propanoate mobility in the active site of myoglobin from Galeorhinus japonicus.
Eur J Biochem. 1990 May 20;189(3):567-73
Authors: Yamamoto Y, Inoue Y, Chûjô R, Suzuki T
Time-dependent NOE studies of the C13(1) and C17(1) methylene proton resonances of the heme...
13C-NMR relaxation study on mobility of the DNA-binding arm of HU.
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