13C APSY-NMR for sequential assignment of intrinsically disordered proteins.
13C APSY-NMR for sequential assignment of intrinsically disordered proteins.
Related Articles 13C APSY-NMR for sequential assignment of intrinsically disordered proteins. J Biomol NMR. 2018 Feb 28;: Authors: Murrali MG, Schiavina M, Sainati V, Bermel W, Pierattelli R, Felli IC Abstract The increasingly recognized biological relevance of intrinsically disordered proteins requires a continuous expansion of the tools for their characterization via NMR spectroscopy, the only technique so far able to provide atomic-resolution information on these highly mobile macromolecules. Here we present the implementation of projection spectroscopy in 13C-direct detected NMR experiments to achieve the sequence specific assignment of IDPs. The approach was used to obtain the complete backbone assignment at high temperature of ?-synuclein, a paradigmatic intrinsically disordered protein. PMID: 29492731 [PubMed - as supplied by publisher] More... |
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