(13)C NMR detection of non-protein nitrogen substance adulteration in animal feed
 

(13)C NMR detection of non-protein nitrogen substance adulteration in animal feed

Illegal adulteration of melamine in animal feed and food has been widely studied. However, the risk of using substitute non-protein nitrogen substances still exists. In this study, we developed the ^(13)C NMR method for the detection of non-protein nitrogen substance adulteration in animal feed. Three compounds, i.e., urea, melamine, and biuret, were used for method development. We found that the chemical shifts of the characteristic peaks in the carbon spectra of high-nitrogen adulterants were...

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