[ASAP] Assessing Site-Specific Enhancements Imparted by Hyperpolarized Water in Folded and Unfolded Proteins by 2D HMQC NMR

		BioNMR > Educational resources > Journal club
[ASAP] Assessing Site-Specific Enhancements Imparted by Hyperpolarized Water in Folded and Unfolded Proteins by 2D HMQC NMR

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

View First Unread

Thread Tools

Search this Thread

Rate Thread

Display Modes

05-12-2020, 08:32 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,169

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

[ASAP] Assessing Site-Specific Enhancements Imparted by Hyperpolarized Water in Folded and Unfolded Proteins by 2D HMQC NMR

[ASAP] Assessing Site-Specific Enhancements Imparted by Hyperpolarized Water in Folded and Unfolded Proteins by 2D HMQC NMR

Or Szekely†?, Gregory Lars Olsen†§, Mihajlo Novakovic†, Rina Rosenzweig‡, and Lucio Frydman*†

Journal of the American Chemical Society
DOI: 10.1021/jacs.0c00807

Source: Journal of the American Chemical Society

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[ASAP] Net Charge and Nonpolar Content Guide the Identification of Folded and Prion Proteins Net Charge and Nonpolar Content Guide the Identification of Folded and Prion Proteins https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.9b01114/20200511/images/medium/bi9b01114_0014.gif Biochemistry DOI: 10.1021/acs.biochem.9b01114 http://feeds.feedburner.com/~r/acs/bichaw/~4/_6_Oea9fEw0 More...	nmrlearner	Journal club	0	05-12-2020 05:10 AM
[NMR paper] Assessing site-specific enhancements imparted by hyperpolarized water in folded and unfolded proteins by 2D HMQC NMR. Assessing site-specific enhancements imparted by hyperpolarized water in folded and unfolded proteins by 2D HMQC NMR. Related Articles Assessing site-specific enhancements imparted by hyperpolarized water in folded and unfolded proteins by 2D HMQC NMR. J Am Chem Soc. 2020 Apr 27;: Authors: Szekely O, Olsen GL, Novakovic M, Rosenzweig R, Frydman L Abstract Dissolution DNP of hyperpolarized water can be a valuable aid in biomolecular NMR. With suitable optimizations one can utilize it to achieve, under near-physiological...	nmrlearner	Journal club	0	04-28-2020 05:29 PM
[ASAP] A Perspective on the Confident Comparison of Glycoprotein Site-Specific Glycosylation in Sample Cohorts A Perspective on the Confident Comparison of Glycoprotein Site-Specific Glycosylation in Sample Cohorts https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.9b00730/20191231/images/medium/bi9b00730_0003.gif Biochemistry DOI: 10.1021/acs.biochem.9b00730 http://feeds.feedburner.com/~r/acs/bichaw/~4/EoEbvgzftqg More...	nmrlearner	Journal club	0	02-29-2020 09:52 PM
Hyperpolarized MAS NMR of unfolded and misfolded proteins #DNPNMR From The DNP-NMR Blog: Hyperpolarized MAS NMR of unfolded and misfolded proteins #DNPNMR König, Anna, Daniel Schölzel, Boran Uluca, Thibault Viennet, Ümit Akbey, and Henrike Heise. “Hyperpolarized MAS NMR of Unfolded and Misfolded Proteins.” Solid State Nuclear Magnetic Resonance 98 (April 2019): 1–11. https://doi.org/10.1016/j.ssnmr.2018.12.003.	nmrlearner	News from NMR blogs	0	03-24-2019 10:41 PM
[NMR paper] High-Resolution NMR of Folded Proteins in Hyperpolarized Physiological Solvents. High-Resolution NMR of Folded Proteins in Hyperpolarized Physiological Solvents. Related Articles High-Resolution NMR of Folded Proteins in Hyperpolarized Physiological Solvents. Chemistry. 2018 Jul 03;: Authors: Kaderavek P, Ferrage F, Bodenhausen G, Kurzbach D Abstract We report hyperpolarized 2D exchange spectroscopy (HYPEX) to obtain high-resolution nuclear magnetic resonance (NMR) spectra of folded proteins under near-physiological conditions. The technique is based on hyperpolarized water, which is prepared by dissolution...	nmrlearner	Journal club	0	07-06-2018 09:40 AM
Site specific polarization transfer from a hyperpolarized ligand of dihydrofolate reductase From The DNP-NMR Blog: Site specific polarization transfer from a hyperpolarized ligand of dihydrofolate reductase p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica} Wang, Y., M. Ragavan, and C. Hilty, Site specific polarization transfer from a hyperpolarized ligand of dihydrofolate reductase. J. Biomol. NMR, 2016. 65(1): p. 41-48. http://dx.doi.org/10.1007/s10858-016-0037-x	nmrlearner	News from NMR blogs	0	05-15-2017 04:34 PM
Site specific polarization transfer from a hyperpolarized ligand of dihydrofolate reductase Site specific polarization transfer from a hyperpolarized ligand of dihydrofolate reductase Abstract Proteinâ??ligand interaction is often characterized using polarization transfer by the intermolecular nuclear Overhauser effect (NOE). For such NOE experiments, hyperpolarization of nuclear spins presents the opportunity to increase the spin magnetization, which is transferred, by several orders of magnitude. Here, folic acid, a ligand of dihydrofolate reductase (DHFR), was hyperpolarized on 1H spins using dissolution dynamic nuclear polarization...	nmrlearner	Journal club	0	05-18-2016 09:53 PM
[NMR paper] NMR characterization of partially folded and unfolded conformational ensembles of pro NMR characterization of partially folded and unfolded conformational ensembles of proteins. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_120x27.gif Related Articles NMR characterization of partially folded and unfolded conformational ensembles of proteins. Biopolymers. 1999;51(3):191-207 Authors: Barbar E Studies of unfolded and partially folded proteins provide important insight into the initiation and process of protein folding. This review focuses on the...	nmrlearner	Journal club	0	08-21-2010 04:03 PM

« Previous Thread | Next Thread »

Thread Tools	Search this Thread
Show Printable Version Email this Page	Search this Thread: Advanced Search
Display Modes	Rate This Thread
Linear Mode Switch to Hybrid Mode Switch to Threaded Mode	Rate This Thread:

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off