Postdoc
Postdoc
Structural studies of Vpx, the accessory protein of the HIV-2 We are looking for a candidate to apply for a 2 years postdoctoral position in the Laboratory of Biological NMR and Crystallography at the University Paris Descartes. The project will aim to determine the structure of the Vpx protein (112 amino acids) and of its complexes with inhibitors identified after screening of small compound libraries, using high-field multidimensional NMR and Molecular Modeling. HIV-2 is the second leading cause of AIDS in humans and we are interested with the Vpx protein that increases the efficiency of infection by lentivectors of various origins, allows the transport of viral DNA to the nucleus and promotes the accumulation of retroviral DNA in some cells of the immune system. Vpx thus allows viral dissemination but its role in the replication of HIV-2 and its molecular mechanism are still not understood. The objectives of this project are to understand at the structural and mechanistic level, how Vpx inhibits cell defenses against viral infection. We aim to identify partners of Vpx in cells and to elucidate its exact role in viral dissemination and its role in the replication of HIV-2. Finally, we want to understand how cellular factors can modulate the early stages of lentiviral infection. The obtaining the Vpx protein structure, identification of partners and the study of their complexes will make our contribution to the understanding of the mechanisms involved in the various activities of this protein. The study of the protein Vpx will be greatly simplified by the experience we have gained from structural and functional studies performed on the Vpr protein. The protein Vpx has been obtained by chemical synthesis and will be produced by overexpression in Coli and 15N labeled. The project will be based on functional and structural experiments that associate liquid-state Nuclear Magnetic Resonance and Molecular Modeling. The candidate should have a good experience in the protein overexpression et their purification in bacterial systems and a good expertise in multidimensional heteronuclear NMR and molecular modeling. The starting date is January 2012 for a two years funding. Application, CV and reference letters should be submitted to: Dr Serge Bouaziz serge.bouaziz@parisdescartes.fr http://lcrbw.pharmacie.univ-paris5.fr/https://blogger.googleusercontent.co...s.blogspot.com More... |
All times are GMT. The time now is 12:19 PM. |
Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.
Search Engine Friendly URLs by vBSEO 3.6.0
Copyright, BioNMR.com, 2003-2013