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NMR processing:
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Side-chains:
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Ab initio:
GeNMR
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Refinement:
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Structure from chemical shifts:
Fragment-based:
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Torsion angles from chemical shifts:
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Secondary structure from chemical shifts:
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Flexibility from chemical shifts:
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Chemical shifts re-referencing:
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RDCs:
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Protein geomtery:
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SAVES2 or SAVES4
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Flexibility from structure:
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Molecular dynamics:
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From structure:
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CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


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Default Genentech Postdoctoral Fellow

Genentech Postdoctoral Fellow

Genentech Postdoctoral Fellow: Structural Biology/NMR Spectroscopy

We have an opportunity for a highly talented Postdoctoral Fellow to join the Protein NMR Group in the Structural Biology Department at Genentech. Our research encompasses the structural analysis of proteins of therapeutic interest, with an emphasis on determining their conformations and ligand-binding properties.

Genentech is now applying our expertise in protein expression, assay development, antibody engineering and drug design to pursue some of the most challenging therapeutic targets in the human proteome, including G protein-coupled receptors and ion channels. The successful candidate will focus their postdoctoral research on the NMR structural analysis of human membrane proteins involved in ion transport and the mechanisms of their functional regulation. The interaction of these proteins with potent and selective antibody fragments, peptides and/or small molecule inhibitors will also be examined. Significant progress on the high-level expression and purification of the proposed membrane structural targets has already been achieved. Moreover, you would be working with a highly collaborative group of scientists whose expertise includes protein NMR spectroscopy, soluble and membrane protein crystallography, ion channel electrophysiology and assay development.

The protein NMR facility includes dedicated 800, 600, and 500 MHz spectrometers, Linux workstations, a fully equipped protein expression and purification lab with expertise in membrane protein production, and access to the Central California 900 MHz spectrometer.
Requirements:

Recent PhD graduate in Biochemistry, Structural Biology or a related discipline, with a strong publication record providing evidence of productive, independent contributions in science. The applicant must have demonstrated expertise in protein expression and purification, and protein NMR spectroscopy . Prior experience working with membrane proteins will be a strong asset, but is not essential. The successful candidate must be motivated, capable of working independently, and enjoy working in a collaborative setting.

Interested candidates should apply at www.gene.com, but feel free to send their CV and cover letter directly to Dr. Melissa Starovasnik (star@gene.com) and Dr. Christopher Koth (kothc@gene.com). Please visit http://www.gene.com/gene/research/postdoctoral for additional information.


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