Solid-state NMR characterization of gas vesicle structure.
Related Articles Solid-state NMR characterization of gas vesicle structure.
Biophys J. 2010 Sep 22;99(6):1932-9
Authors: Sivertsen AC, Bayro MJ, Belenky M, Griffin RG, Herzfeld J
Gas vesicles are gas-filled buoyancy organelles with walls that consist almost exclusively of gas vesicle protein A (GvpA). Intact, collapsed gas vesicles from the cyanobacterium Anabaena flos-aquae were studied by solid-state NMR spectroscopy, and most of the GvpA sequence was assigned. Chemical shift analysis indicates a coil-α-β-β-α-coil peptide backbone, consistent with secondary-structure-prediction algorithms, and complementary information about mobility and solvent exposure yields a picture of the overall topology of the vesicle subunit that is consistent with its role in stabilizing an air-water interface.
PMID: 20858439 [PubMed - in process]
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PubMed