Thread: NMR paper Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution.
View Single Post
  #1  
Unread 08-22-2010, 03:03 PM
nmrlearner's Avatar
nmrlearner nmrlearner is offline
Senior Member
  
Join Date: Jan 2005
Posts: 23,173
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 0
Downloads: 0
Uploads: 0
Default Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution.
Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution.

Related Articles Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution.

Science. 1997 May 9;276(5314):930-2

Authors: Hall DA, Maus DC, Gerfen GJ, Inati SJ, Becerra LR, Dahlquist FW, Griffin RG

Large dynamic nuclear polarization signal enhancements (up to a factor of 100) were obtained in the solid-state magic-angle spinning nuclear magnetic resonance (NMR) spectra of arginine and the protein T4 lysozyme in frozen glycerol-water solutions with the use of dynamic nuclear polarization. Polarization was transferred from the unpaired electrons of nitroxide free radicals to nuclear spins through microwave irradiation near the electron paramagnetic resonance frequency. This approach may be a generally applicable signal enhancement scheme for the high-resolution solid-state NMR spectroscopy of biomolecules.

PMID: 9139651 [PubMed - indexed for MEDLINE]



Source: PubMed
Reply With Quote

Did you find this post helpful? Yes | No