Comparative 1H-NMR studies on the physical state of water in soft contact lens and mouse lens.
Related Articles Comparative 1H-NMR studies on the physical state of water in soft contact lens and mouse lens.
Biochim Biophys Acta. 1996 Apr 17;1289(3):369-76
Authors: Kuwata K, Era S, Sogami M, Amano H, Nagaoka S, Kato K, Takahashi K, Kitazawa Y, Watari H
The physical state of water in mouse lenses (2-, 4- or 8-wk-old) and soft contact lenses (SCLs, water content from 18.4 to 79.2%) were studied by measuring spin-lattice relaxation times (T1) and apparent intermolecular cross-relaxation times (TIS) from irradiated protein or polymer protons to water protons, using 360 MHz 1H-NMR spectrometer at 25 degrees C. (1) 1/T1 values of SCLs increased gradually with increasing dry weight (W(%)). 1/TIS values of SCLs were approximately zero at W of 20.8 and 26.8%, increased gradually from 26.8% and then steeply above approximately 50%. (2) A plot of 1/T1 vs. W(%) of mouse lenses was almost equal to that of SCLs. However, a plot of 1/TIS vs. W(%) was an approximately straight line with the intercept at W of 23% and with the slope which is almost equal to that of SCLs above W of approximately 50%. The plot of 1/TIS vs. W(%) of mouse lenses might indicate the significant change in the physical state of water and/or protein-water interactions above W of 23%.
PMID: 8620021 [PubMed - indexed for MEDLINE]
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PubMed