Thread: NMR paper H-NMR studies of native and fragmented subtilisin Carlsberg.
View Single Post
  #1  
Unread 08-21-2010, 11:41 PM
nmrlearner's Avatar
nmrlearner nmrlearner is offline
Senior Member
  
Join Date: Jan 2005
Posts: 23,175
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 0
Downloads: 0
Uploads: 0
Default H-NMR studies of native and fragmented subtilisin Carlsberg.
H-NMR studies of native and fragmented subtilisin Carlsberg.

Related Articles H-NMR studies of native and fragmented subtilisin Carlsberg.

Biochim Biophys Acta. 1992 Feb 13;1119(1):39-44

Authors: Consonni R, Molinari H, Greco F, Zannoni G, Zetta L, Carrea G, Riva S

NMR studies have been carried out on subtilisin Carlsberg in order to identify the sharp resonances observed in the proton spectra of the enzyme dissolved in aqueous solution. NMR spectra, obtained with the combination of spin-echo and selective excitation sequences, from both the native and inactivated protein, enabled us to assign sharp signals to subtilisin fragments derived from enzymatic autolysis (monitored by high-performance size-exclusion chromatography), rather than to mobile segments of the intact protein.

PMID: 1540633 [PubMed - indexed for MEDLINE]



Source: PubMed
Reply With Quote

Did you find this post helpful? Yes | No