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Default Identification of the single-stranded DNA binding surface of the transcriptional coac

Identification of the single-stranded DNA binding surface of the transcriptional coactivator PC4 by NMR.

Related Articles Identification of the single-stranded DNA binding surface of the transcriptional coactivator PC4 by NMR.

J Biol Chem. 1999 Feb 5;274(6):3693-9

Authors: Werten S, Wechselberger R, Boelens R, van der Vliet PC, Kaptein R

The C-terminal domain of the eukaryotic transcriptional cofactor PC4 (PC4CTD) is known to bind with nanomolar affinity to single-stranded (ss)DNA. Here, NMR is used to study DNA binding by this domain in more detail. Amide resonance shifts that were observed in a 1H15N-HSQC-monitored titration of 15N-labeled protein with the oligonucleotide dT18 indicate that binding of the nucleic acid occurs by means of two anti-parallel channels that were previously identified in the PC4CTD crystal structure. The beta-sheets and loops that make up these channels exhibit above average flexibility in the absence of ssDNA, which is reflected in higher values of T1rho, reduced heteronuclear nuclear Overhauser effects and faster deuterium exchange rates for the amides in this region. Upon ssDNA binding, this excess flexibility is significantly reduced. The binding of ssDNA by symmetry-related channels reported here provides a structural rationale for the preference of PC4CTD for juxtaposed single-stranded regions (e.g. in heteroduplexes) observed in earlier work.

PMID: 9920920 [PubMed - indexed for MEDLINE]



Source: PubMed
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