Thread: How can one determine quantity of an organic molecule by its NMR spectra?
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Unread 08-17-2004, 04:21 PM
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Default how to you determine the quantity of an organic molecule by its NMR spectra?
You need to have a calibration curve or at least a comparison.The area of a peak (the integration) in NMR is quantitative, that is, the more sample you have, the bigger the are would be. just like how the area of signal of 1 proton is smaller than the signal of 2 proton.So you just have to compare the the integration of a specific peak of your sample to the standard or to the standard calibration curve"The most important fundamental relation of qNMR is that the signal response (integrated signal area) Ix in a spectrum is directly proportional to the number nuclei Nx generating the corresponding resonance line:Ix = KsNxwith Ks as a spectrometer constant"Journal of Pharmaceutical and Biomedical Analysis 38 (2005) 813–823
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