Thread: NMR pulse sequences of Danish Instrument Center
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Unread 03-16-2005, 07:39 AM
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The following NMR pulse sequences for biological macromolecules are available on the website of the Danish Instrument Center as of 3/15/05:
  • Coupling Constants in Proteins
    - 2D S3CT for measurement of one bond coupling constants (J & RDC) - Varian, Bruker
  • TROSY experiments
    - clean TROSY, 15N-1H correlation, gradient version, water flip back, optional t2 decoupling - Varian, Bruker
    - constant-time S3CT TROSY, 13 C-1H correlation, gradient version - Varian, Bruker
    - (H)CCH TROSY, 3D TROSY-type HCCH correlation with diagonal peak suppression - Varian
    - TROSY-Type NOESY, 2D NOESY with amide diagonal peak suppression - Varian
    - TROSY-NOESY, 3D version with amide diagonal peak suppression - Varian, Bruker
    - HNCA, 3D sequential HNCA for Protein Backbone Assignment - Varian, Bruker
    - HNCACB, 3D sequential HNCACB for Protein Backbone Assignment - Varian
  • J Coupling Constants across Hydrogen Bonds
    - 3D S3(HN) TROSY hHNCO pulse sequence for measurement of 2hJ(HN,CO) and 3hJ(N,CO) - Varian
    - 3D C E.COSY TROSY hHNCO pulse sequence for measurement of 3hJ(HN,C) - Varian
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