Evaluation of the tert-butyl group as a probe for NMR studies of macromolecular complexes
The development of methyl transverse relaxation optimized spectroscopy has greatly facilitated the study of macromolecular assemblies by solution NMR spectroscopy. However, limited sample solubility and stability has hindered application of this technique to ongoing studies of complexes formed on membranes by the neuronal SNAREs that mediate neurotransmitter release and synaptotagmin-1, the Ca^(2+) sensor that triggers release. Since the ¹H NMR signal of a ^(t)Bu group attached to a large...
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