The Incorporation of Labile Protons into Multidimensional NMR Analyses: Glycan Structures Revisited
Glycan structures are often stabilized by a repertoire of hydrogen-bonded donor/acceptor groups, revealing longer-lived structures that could represent biologically relevant conformations. NMR provides unique data on these hydrogen-bonded networks from multidimensional experiments detecting cross-peaks resulting from through-bond (TOCSY) or through-space (NOESY) interactions. However, fast OH/H(2)O exchange, and the spectral proximity among these NMR resonances, hamper the use of glycans' labile...
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