From The DNP-NMR Blog:
New limits of sensitivity of site-directed spin labeling electron paramagnetic resonance for membrane proteins #DNPNMR
A nice overview how spinlabels can be used for structural biology studies. This includes pulsed EPR techniques such as PELDOR (DEER) and ODNP spectroscopy.
Bordignon, Enrica, and Stephanie Bleicken. “New Limits of Sensitivity of Site-Directed Spin Labeling Electron Paramagnetic Resonance for Membrane Proteins.” Biochimica et Biophysica Acta (BBA) - Biomembranes 1860, no. 4 (April 2018): 841–53.
https://doi.org/10.1016/j.bbamem.2017.12.009.
Site-directed spin labeling electron paramagnetic resonance is a biophysical technique based on the specific introduction of spin labels to one or more sites in diamagnetic proteins, which allows monitoring dynamics and water accessibility of the spin-labeled side chains, as well as nanometer distances between two (or more) labels. Key advantages of this technique to study membrane proteins are addressed, with focus on the recent developments which will expand the range of applicability. Comparison with other biophysical methods is provided to highlight the strength of EPR as complementary tool for structural biology. This article is part of a Special Issue entitled: Beyond the Structure-Function Horizon of Membrane Proteins edited by Ute Hellmich, Rupak Doshi and Benjamin McIlwain.
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