RNA dynamics by NMR.
Related Articles RNA dynamics by NMR.
Chembiochem. 2019 Apr 17;:
Authors: Maruši? M, Schlagnitweit J, Petzold K
Abstract
An ever-increasing number of functional RNAs require mechanistic understanding. RNA function relies on changes in its structure, so-called dynamics. To reveal dynamic processes and higher energy structures, new Nuclear Magnetic Resonance (NMR) methods have been developed to elucidate these dynamics in RNA with atomic resolution. In this review, we provide an introduction to dynamics novices and an overview of methods that access most dynamic timescales, from picoseconds to hours. Examples are provided as well as insight into
theory, data acquisition and analysis for these different methods. Using this broad spectrum of methodology, unprecedented details and invisible structures have been obtained and are reviewed here. RNA, though often more complicated and therefore neglected, also provides a great system to study structural changes, as RNA structural changes are easier definable than in proteins, hence the numerous revelations of RNA excited states.
PMID: 30997719 [PubMed - as supplied by publisher]
More...