Solution NMR Structure and Backbone Dynamics of Recombinant Bee Venom Melittin.
Related Articles Solution NMR Structure and Backbone Dynamics of Recombinant Bee Venom Melittin.
Biochemistry. 2018 Apr 18;:
Authors: Ramirez L, Shekhtman A, Pande J
Abstract
In recent years there has been a resurgence of interest in melittin and its variants as their therapeutic potential has become increasingly evident. Melittin is a 26-residue peptide and a toxic component of honey bee venom. The versatility of melittin in interacting with various biological substrates, such as membranes, glycosaminoglycans and a variety of proteins has inspired a slew of studies to understand the structural basis of such interactions. However, these studies have largely focused on melittin solutions at high concentrations (> 1mM), even though melittin is generally effective at lower, (micromolar) concentrations. Here we present high-resolution NMR studies in the lower concentration regime using a novel method to produce isotope labeled (15N, 13C) recombinant melittin. We provide residue-specific structural characterization of melittin in dilute aqueous solution and in TFE-water mixtures, which mimic melittin structure-function and interactions in aqueous, and membrane-like environments respectively. We find that the cis-trans isomerization of Pro14 is key to changes in the secondary structure of melittin. Thus, this study provides residue-specific structural information on melittin in the free-state and in a model of the substrate-bound state. These results, taken together with published work from other labs, reveal the peptide's structural versatility which resembles that of intrinsically disordered proteins and peptides.
PMID: 29668274 [PubMed - as supplied by publisher]
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