NMR-Based Metabolomics of Oral Biofluids.
Related Articles NMR-Based Metabolomics of Oral Biofluids.
Methods Mol Biol. 2017;1537:79-105
Authors: Schirra HJ, Ford PJ
Abstract
NMR-based metabolomics is an established technique for characterizing the metabolite profile of biological fluids and investigating how metabolite profiles change in response to biological and/or clinical stimuli. Thus, NMR-based metabolomics has the potential to discover biomarkers for diagnosis, prognosis, and/or therapy of clinical conditions, as well as to unravel the physiology underlying clinical conditions. Here, we describe a detailed protocol for NMR-based metabolomics of oral biofluids, including sample collection, sample handling, NMR data acquisition, and processing. In addition, we give a general overview of the statistical analysis of the resulting metabolomic data.
PMID: 27924589 [PubMed - indexed for MEDLINE]
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