An adaptable phospholipid membrane mimetic system for solution NMR studies of membrane proteins.
An adaptable phospholipid membrane mimetic system for solution NMR studies of membrane proteins.
J Am Chem Soc. 2017 Oct 09;:
Authors: Chien CH, Helfinger LR, Bostock MJ, Solt A, Tan YL, Nietlispach D
Abstract
Based on the saposin-A (SapA) scaffold protein we demonstrate the suitability of a size-adaptable phospholipid membrane-mimetic system for solution NMR studies of membrane proteins under close-to-native conditions. The Salipro nanoparticle size can be tuned over a wide pH range by adjusting the saposin-to-lipid stoichiometry enabling maintenance of sufficiently high amounts of phospholipid in the Salipro nanoparticle to mimic a realistic membrane environment while controlling the overall size to enable solution NMR for a range of membrane proteins. Three representative membrane proteins including one G-protein-coupled receptor (GPCR) were successfully incorporated into SapA-dimyristoylphosphatidylcholine (DMPC) nanoparticles and studied by solution NMR spectroscopy.
PMID: 28990386 [PubMed - as supplied by publisher]
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