Combining DNP NMR with segmental and specific labeling to study a yeast prion protein strain that is not parallel in-register [Biophysics and Computational Biology]
Kendra K. Frederick, Vladimir K. Michaelis, Marc A. Caporini, Loren B. Andreas, Galia T. Debelouchina, Robert G. Griffin, Susan Lindquist...
Date: 2017-04-04
The yeast prion protein Sup35NM is a self-propagating amyloid. Despite intense study, there is no consensus on the organization of monomers within Sup35NM fibrils. Some studies point to a ?-helical arrangement, whereas others suggest a parallel in-register organization. Intermolecular contacts are often determined by experiments that probe long-range heteronuclear contacts...
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PNAS:
Number: 14
Volume: 114