Thread: NMR paper Measurement of Ligand-Target Residence Times by (1)H Relaxation Dispersion NMR Spectroscopy.
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Default Measurement of Ligand-Target Residence Times by (1)H Relaxation Dispersion NMR Spectroscopy.
Measurement of Ligand-Target Residence Times by (1)H Relaxation Dispersion NMR Spectroscopy.

Related Articles Measurement of Ligand-Target Residence Times by (1)H Relaxation Dispersion NMR Spectroscopy.

J Med Chem. 2016 Dec 08;59(23):10788-10793

Authors: Moschen T, Grutsch S, Juen MA, Wunderlich CH, Kreutz C, Tollinger M

Abstract
A ligand-observed (1)H NMR relaxation experiment is introduced for measuring the binding kinetics of low-molecular-weight compounds to their biomolecular targets. We show that this approach, which does not require any isotope labeling, is applicable to ligand-target systems involving proteins and nucleic acids of variable molecular size. The experiment is particularly useful for the systematic investigation of low affinity molecules with residence times in the micro- to millisecond time regime.


PMID: 27933946 [PubMed - in process]



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