Characterization of fibril dynamics on three timescales by solid-state NMR
Abstract
A multi-timescale analysis of the backbone dynamics of HET-s (218â??289) fibrils is described based on multiple site-specific
R 1 and
R 1Ï? data sets and
S 2 measurements via REDOR for most backbone 15N and 13Cα nuclei. 15N and 13Cα data are fitted with motions at three timescales. Slow motion is found, indicating a global fibril motion. We further investigate the effect of 13Câ??13C transfer in measurement of 13Cα
R 1. Finally, we show that it is necessary to go beyond the Redfield approximation for slow motions in order to obtain accurate numerical values for
R 1Ï?.
Source: Journal of Biomolecular NMR