Analysis of Interaction of Estradiol with Estrogen Receptor by NMR Spectroscopy.
Related Articles Analysis of Interaction of Estradiol with Estrogen Receptor by NMR Spectroscopy.
Methods Mol Biol. 2016;1366:233-9
Authors: Thakur MK, Paramanik V
Abstract
Following binding to estradiol, estrogen receptors (ER) ? and ER? recruit a number of interacting proteins and mediate a plethora of functions. The binding of estrogen with the receptors shows changes in the resonance structure and movement of protons. We cloned ER? and its trans-activation domain (TAD) and ligand-binding domain (LBD), expressed them in prokaryotic expression vectors, purified them, and studied their interaction with estradiol. In this chapter, a detailed method of preparation of recombinant proteins, SDS-PAGE, silver staining, and NMR are described. Such methods are useful to check the biological activity of bacterially expressed proteins and are applicable to basic and applied research.
PMID: 26585139 [PubMed - in process]
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