Magic angle spinning NMR of viruses
Publication date: April 2015
Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volumes 86–87
Author(s): Caitlin M. Quinn , Manman Lu , Christopher L. Suiter , Guangjin Hou , Huilan Zhang , Tatyana Polenova
Viruses, relatively simple pathogens, are able to replicate in many living organisms and to adapt to various environments. Conventional atomic-resolution structural biology techniques, X-ray crystallography and solution NMR spectroscopy provided abundant information on the structures of individual proteins and nucleic acids comprising viruses; however, viral assemblies are not amenable to analysis by these techniques because of their large size, insolubility, and inherent lack of long-range order. In this article, we review the recent advances in magic angle spinning NMR spectroscopy that enabled atomic-resolution analysis of structure and dynamics of large viral systems and give examples of several exciting case studies.
Graphical abstract
More...