Bringing Dynamic Molecular Machines into Focus by Methyl-TROSY NMR.
Related Articles Bringing Dynamic Molecular Machines into Focus by Methyl-TROSY NMR.
Annu Rev Biochem. 2014 Jun 2;83:291-315
Authors: Rosenzweig R, Kay LE
Abstract
Large macromolecular assemblies, so-called molecular machines, are critical to ensuring proper cellular function. Understanding how proper function is achieved at the atomic level is crucial to advancing multiple avenues of biomedical research. Biophysical studies often include X-ray diffraction and cryo-electron microscopy, providing detailed structural descriptions of these machines. However, their inherent flexibility has complicated an understanding of the relation between structure and function. Solution NMR spectroscopy is well suited to the study of such dynamic complexes, and continued developments have increased size boundaries; insights into function have been obtained for complexes with masses as large as 1 MDa. We highlight methyl-TROSY (transverse relaxation optimized spectroscopy) NMR, which enables the study of such large systems, and include examples of applications to several cellular machines. We show how this emerging technique contributes to an understanding of cellular function and the role of molecular plasticity in regulating an array of biochemical activities.
PMID: 24905784 [PubMed - as supplied by publisher]
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