Thread: NMR paper Quantitation of Recombinant Protein in Whole Cells and Cell Extracts with Solid-State NMR Spectroscopy.
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Default Quantitation of Recombinant Protein in Whole Cells and Cell Extracts with Solid-State NMR Spectroscopy.
Quantitation of Recombinant Protein in Whole Cells and Cell Extracts with Solid-State NMR Spectroscopy.

Related Articles Quantitation of Recombinant Protein in Whole Cells and Cell Extracts with Solid-State NMR Spectroscopy.

Biochemistry. 2013 Jun 6;

Authors: Vogel EP, Weliky DP

Abstract
Recombinant proteins (RPs) are commonly expressed in bacteria followed by solubilization and chromatography. Purified RP yield can be diminished by losses at any step with very different changes in methods needed to try to improve yield. Time and labor can therefore be saved by first identifying the specific reason for low yield. The present study describes a new solid-state NMR approach to RP quantitation in whole cells or cell pellets without solubilization or purification. The approach is straightforward and inexpensive and only requires ~50 mL culture and a low-field spectrometer.


PMID: 23742073 [PubMed - as supplied by publisher]



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