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Unread 12-11-2012, 07:30 PM
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Default why we are seeing negative PREs in paramagnetic relaxation enhancement experiment?

why we are seeing negative PREs in paramagnetic relaxation enhancement experiment?

Hi NMR Wiki users,

I'm using two point method(Marius Clore and Junji Iwahara methods) to measure the PREs, but in my experiment I observed so many negative PREs. Is there any chance to use these negative PREs data in structure calculation.How can I get all positive PREs,otherwise I lose half of the data in my calculations.



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