residue information in case of line broadening (intensity drop ) of HSQC titration
Dear Friends
we have done HSQC gradient titration with targeted proteins , most of cross peaks we observed intensity drop , 30 out of 95 peaks ( 70 percent intensity drop of each cross peak ) , other cross peaks also we observe intensity drop less than 70 percent , we did n"t observe any chemical shift change .( May be it is due to intermediate exchange regime of complex ) .ITC experiment showing 12uM binding constant .AUC experiment result is complex size 33 kd .
is their any method to extract residue information from intensity drop of HSQC titration spectra more specifically ?
How to minimize line broadening effect while taking HSQC Titration ?
Check if somebody has answered this question on
NMRWiki QA forum