Thread: Question from NMRWiki Q&A forum HSQC perturbation
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Unread 02-14-2012, 03:00 AM
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Default HSQC perturbation
HSQC perturbation

Dear All,

I am trying to determine the interaction between 2 proteins. Is there any reference or literature which report the determination of binding constant between 2 proteins in which intensity drop of HSQC cross peaks can be used as the measuring parameter. As when I titrate these 2 proteins only change which I observe is intensity drop. I tried other biophysical techniques to determine binding constant, but results are not satisfactory.

Any suggestion would be useful

Regards

Arun



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