Wheat Germ Cell-Free Protein Production Workshop
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The Center for Eukaryotic Structural Genomics (CESG) and the Nuclear Magnetic Resonance Facility at Madison
(NMRFAM) are pleased to announce the first Wheat Germ Cell-Free Protein Production Workshop to be held from
July 30 - August 4, 2006, at the Department of Biochemistry at the University of Wisconsin-Madison in Madison, Wisconsin,
USA. To register for this workshop, complete the Registration Form on the next page and mail in with your NONREFUNDABLE
payment of $500.00 (academic) or $750.00 (private industry) by May 15, 2006.
PURPOSE
The workshop will offer participants a unique opportunity to obtain hands-on experience with cell-free protein production
from wheat germ extracts. The week-long workshop will cover various aspects of small-scale expression screening (μg
quantities of attendee-nominated target protein) and large-scale production (mg quantities of target protein selected from
attendee efforts) of labeled proteins for NMR spectroscopy or crystallization screening. Both manual and automated
procedures will be covered.
SCOPE
Participants can try these approaches with their own targets. Necessary protocols will be sent to all participants just prior to
the workshop. All the data generated during the workshop by all of the participants as well as any relevant presentation and
seminar materials will be sent to all of the participants after the completion of the workshop. The workshop will be limited to
24 participants and geared toward scientists with at least 3-5 years of experience in protein production and purification. A
registration fee will be required to help defray reagent costs.
TRAVEL AND LODGING
Travel and lodging need be arranged by each participant. We have a block of rooms set aside that you can reserve for a
reduced rate ($89 for one person or $99 for two people per night) at the Best Western InnTowner (
www.inntowner.com).
When you make your reservation, ask for a room in the "CESG block." NOTE: This block of rooms will only be available
until June 1, 2006. This hotel was chosen because it is very convenient to CESG and NMRFAM. They have a free shuttle
which will bring you right to the workshop location, or one can walk the distance in about 15 minutes.
REGISTRATION
Your registration fee of $500.00 (academic) or $750.00 (private industry) includes the workshop fees, dinner at the opening and
closing receptions, and continental breakfast, lunch buffets, and afternoon breaks every day. The preferred method for payment of
your registration fee is by credit card, but can also be made in U.S. funds, drawn on a U.S. bank, or by international money order
payable to "University of Wisconsin-Madison." Your registration fee is NON-REFUNDABLE, and the registration deadline for the
workshop is May 15, 2006. Participation in the workshop will be limited to a total of 24 participants, so we encourage those
who are interested to register early. Instructional materials (protocols, links to reference information, and data) will be sent
to participants in advance of the workshop. If you have any questions, send an e-mail to:
cesginfo@biochem.wisc.edu.