Thread: A supplementary coil forH decoupling with commercial HCN MAS probes
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Unread 10-22-2011, 10:16 AM
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Default A supplementary coil forH decoupling with commercial HCN MAS probes
A supplementary coil forH decoupling with commercial HCN MAS probes


Publication year: 2011
Source: Journal of Magnetic Resonance, Available online 20 October 2011

Matthias*Huber, Oliver*With, Paul*Schanda, René*Verel, Matthias*Ernst, ...

Partial deuteration is a powerful tool to increase coherence life times and spectral resolution in proton solid-state NMR. The J coupling to deuterium needs, however, to be decoupled to maintain the good resolution in the (usually indirect)C dimension(s). We present a simple and reversible way to expand a commercial 1.3mm HCN MAS probe with aH channel with sufficient field strength for scalar deuterium decoupling, namely 2-3 kHz. The coil is placed at the outside of the stator and requires no significant modifications to the probe. The performance and the realizable gains in sensitivity and resolution are demonstrated using perdeuterated ubiquitin, with selectively CHD2-labeled methyl groups.

Graphical abstract



Highlights

? Deuterium decoupling leads to enhanced resolution in perdeuterated proteins. ? We describe a reversible modification for a commercial probehead for 2H decoupling. ? Peak intensity increases by a factor of 2.4 in ubiquitin.



Source: Journal of Magnetic Resonance
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