Nanodiscs vs. Macrodiscs for NMR of Membrane Proteins.
Nanodiscs vs. Macrodiscs for NMR of Membrane Proteins.
Biochemistry. 2011 Sep 21;
Authors: Park SH, Berkamp S, Cook GA, Chan MK, Viadiu H, Opella SJ
Abstract
It is challenging to find membrane mimics that stabilize the native structure, dynamics, and functions of membrane proteins. In a recent advance, nanodiscs have been shown to provide a bilayer environment compatible with solution NMR. Increasing the lipid to "belt" peptide ratio expands their diameter, slows their reorientation rate, and enables the protein-containing discs to be aligned in a magnetic field for Oriented Sample solid-state NMR. Here, we compare the spectroscopic properties of membrane proteins with between one and seven trans-membrane helices in q=0.1 isotropic bicelles, ~10 nm diameter isotropic nanodiscs, ~30 nm diameter magnetically aligned macrodiscs, and q=5 bicelles.
PMID: 21936505 [PubMed - as supplied by publisher]
Source:
PubMed