Overcoming the solubility limit with solubility-enhancement tags: successful applications in biomolecular NMR studies
Abstract Although the rapid progress of NMR technology has significantly expanded the range of NMR-trackable systems, preparation of NMR-suitable samples that are highly soluble and stable remains a bottleneck for studies of many biological systems. The application of solubility-enhancement tags (SETs) has been highly effective in overcoming solubility and sample stability issues and has enabled structural studies of important biological systems previously deemed unapproachable by solution NMR techniques. In this review, we provide a brief survey of the development and successful applications of the SET strategy in biomolecular NMR. We also comment on the criteria for choosing optimal SETs, such as for differently charged target proteins, and recent new developments on NMR-invisible SETs.
- Content Type Journal Article
- Pages 23-31
- DOI 10.1007/s10858-009-9371-6
- Authors
- Pei Zhou, Duke University Medical Center Department of Biochemistry 242 Nanaline Duke Building, Research Drive Durham NC 27710 USA
- Gerhard Wagner, Harvard Medical School Department of Biological Chemistry and Molecular Pharmacology Building C1, Room 112, 240 Longwood Avenue Boston MA 02115 USA
Source: Journal of Biomolecular NMR