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Unread 09-15-2005, 04:19 AM
alan.robot alan.robot is offline
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Yes, I read the same paper and tried it with favorable results. I am working on a ~10kDa RNA binding protein from Drosophila which would precipitate after only 3 days in the NMR. After optimizing the buffer conditions it would last ~ a week but still slowly precipitate. I tried 50mM Arg/Glu and now my sample is happy for several weeks! Still have really bad s/n because of all the salt, though (if I go lower than 100mM KCl it aggregates, and the Arg-HCl and K+ Glu salts are really driving up the effective salt concentration). All that and parts of protein seem to be exchange-broadened But at least it lasts long enough to do most triple resonance experiments now.


Cheers,
Alan

P.S. Like the paper says it's important to add the arg/glu when the sample is fairly dilute, that is before the final concentration. Also, prepare some D2O with 50mM arg/glu to avoid cloudiness when adding the 10% to your sample.
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