Techniques and applications of NMR to membrane proteins.
Related Articles Techniques and applications of NMR to membrane proteins.
Mol Membr Biol. 2004 May-Jun;21(3):129-41
Authors: Nielsen N, Malmendal A, Vosegaard T
The fact that membrane proteins are notoriously difficult to analyse using standard protocols for atomic-resolution structure determination methods have motivated adaptation of these techniques to membrane protein studies as well as development of new technologies. With this motivation, liquid-state nuclear magnetic resonance (NMR) has recently been used with success for studies of peptides and membrane proteins in detergent micelles, and solid-state NMR has undergone a tremendous evolution towards characterization of membrane proteins in native membrane and oriented phospholipid bilayers. In this mini-review, we describe some of the technological challenges behind these efforts and provide examples on their use in membrane biology.
PMID: 15204621 [PubMed - indexed for MEDLINE]
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PubMed